Microglia are the main source of productive infection by human immunodeficiency virus type 1 (HIV-1) in the central nervous system (CNS). Infection of microglia is difficult to study because nonhuman microglia are not infected by HIV-1, adult human microglia from surgically removed brain tissues are scarce, and fetal humanCited by: Microglia are embryonically seeded macrophages that contribute to brain development, homeostasis, and pathologies. It is thus essential to decipher how microglial properties are temporally regulated by intrinsic and extrinsic factors, such as sexual identity and the facialxx.xyz by:
The use of adult cells is a suitable alternative to the use of neonatal brain microglia, as many pathologies studied mainly affect the postnatal spinal cord. These culture systems are also useful for directly testing the effect of compounds that may either inhibit or promote microglial facialxx.xyz by: This protocol is an efficient, cost effective and robust method of isolating primary microglia from live, adult, human brain tissue. Isolated primary human microglia can serve as a tool for studying cellular processes in homeostasis and facialxx.xyz: Ishan Agrawal, Shivanjali Saxena, Preethika Nair, Deepak Jha, Sushmita Jha.
Microglia play key roles in regulating synapse development and refinement in the developing brain, but it is unknown whether they are similarly involved during adult neurogenesis. By transiently depleting microglia from the healthy adult mouse brain, we show that microglia are necessary for . Mar 09, · Microglia regulate adult neurogenesis in the subgranular zone of the DG through ongoing phagocytosis of apoptotic neuroblasts (Sierra et al., ), although they do not seem to be similarly involved in the subventricular zone (SVZ) or rostral migratory stream (RMS) (Kyle et al., ; but see Ribeiro Xavier et al., for counterevidence).Cited by: 6.